Ultra-High Resolution 3D Imaging of Whole Cells
Fang Huang and George Sirinakis and Edward S. Allgeyer and Lena K. Schroeder and Whitney C. Duim and Emil B. Kromann and Thomy Phan and Felix E. Rivera-Molina and Jordan R. Myers and Irnov Irnov and Mark Lessard and Yongdeng Zhang and Mary Ann Handel and Christine Jacobs-Wagner and C. Patrick Lusk and James E. Rothman and Derek Toomre and Martin J. Booth and Joerg Bewersdorf.Cell, pages 1028--1040, 2016.
[abstract] [bibtex] [doi] [pdf]
Fluorescence nanoscopy, or super-resolution microscopy, has become an important tool in cell biological research. However, because of its usually inferior resolution in the depth direction (50–80 nm) and rapidly deteriorating resolution in thick samples, its practical biological application has been effectively limited to two dimensions and thin samples. Here, we present the development of whole-cell 4Pi single-molecule switching nanoscopy (W-4PiSMSN), an optical nanoscope that allows imaging of three-dimensional (3D) structures at 10- to 20-nm resolution throughout entire mammalian cells. We demonstrate the wide applicability of W-4PiSMSN across diverse research fields by imaging complex molecular architectures ranging from bacteriophages to nuclear pores, cilia, and synaptonemal complexes in large 3D cellular volumes.
@article{ huangCell16,
author = "Fang Huang and George Sirinakis and Edward S. Allgeyer and Lena K. Schroeder and Whitney C. Duim and Emil B. Kromann and Thomy Phan and Felix E. Rivera-Molina and Jordan R. Myers and Irnov Irnov and Mark Lessard and Yongdeng Zhang and Mary Ann Handel and Christine Jacobs-Wagner and C. Patrick Lusk and James E. Rothman and Derek Toomre and Martin J. Booth and Joerg Bewersdorf",
title = "Ultra-High Resolution 3D Imaging of Whole Cells",
year = "2016",
abstract = "Fluorescence nanoscopy, or super-resolution microscopy, has become an important tool in cell biological research. However, because of its usually inferior resolution in the depth direction (50–80 nm) and rapidly deteriorating resolution in thick samples, its practical biological application has been effectively limited to two dimensions and thin samples. Here, we present the development of whole-cell 4Pi single-molecule switching nanoscopy (W-4PiSMSN), an optical nanoscope that allows imaging of three-dimensional (3D) structures at 10- to 20-nm resolution throughout entire mammalian cells. We demonstrate the wide applicability of W-4PiSMSN across diverse research fields by imaging complex molecular architectures ranging from bacteriophages to nuclear pores, cilia, and synaptonemal complexes in large 3D cellular volumes.",
url = "https://www.cell.com/fulltext/S0092-8674(16)30745-0",
eprint = "https://www.cell.com/cell/pdfExtended/S0092-8674(16)30745-0",
journal = "Cell",
pages = "1028--1040",
doi = "https://doi.org/10.1016/j.cell.2016.06.016"
}